|For Research Use Only
Animal Component Free Cryopreservation medium
Cryo-Ess® (Cryo-preservation medium) is used as a cryopreservation solution without addition of cryoprotective agents (e.g. DMSO. HES).
Using a controlled method of freezing tissues or cells, they can be stored down to -196° in Cryo-Ess® without adding DMSO, where they are maintained in a vital state. Crystallization of liquid in the tissue or cells is minimized in Cryo-Ess® during thawing (which is critical for cell viability). Therefore tissue and cells in the tissue will remain vital in thawing in Cryo-Ess®. Jurkat Cells Frozen in Cryo-Ess® Freezing Medium
|Results of two independent freezing experiments. Jurkat cells were frozen in CryoEss® freezing medium and is control in 90% cell culture medium + 10% DMSO (standard). Directly after thawing the cells were counted and the viability was measured by the automated cell counter CASY TT (direct viability).